Study with ASCP-MLT Actual Test Questions
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Updated: Jul 18, 2026
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ASCP ASCP-MLT Exam Overview:
| Certification Vendor: | ASCP Board of Certification (ASCP BOC) |
| Exam Name: | MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) |
| Exam Number: | ASCP-MLT |
| Exam Price: | $235 USD (standard); $215 USD (reduced for eligible regions) |
| Real Exam Qty: | 100 |
| Exam Duration: | 150 minutes |
| Exam Format: | Computer Adaptive Testing (CAT), Multiple-choice questions |
| Available Languages: | English |
| Passing Score: | 400 (scale 0-999) |
| Related Certifications: | MLT(ASCPi) MLS(ASCP) PBT(ASCP) |
| Certificate Validity Period: | 3 years (renewable via CMP) |
| Recommended Training: | ASCP Recommended Reading List ASCP BOC MLT Content Guideline |
| Exam Registration: | Pearson VUE Testing Center Scheduling ASCP BOC Official Application Portal |
| Sample Questions: | ASCP ASCP-MLT Sample Questions |
| Exam Way: | Proctored computer-based testing at authorized Pearson VUE test centers |
| Pre Condition: | Associate degree or 60 semester hours from accredited institution + completion of NAACLS/ABHES-accredited MLT program OR approved education + clinical experience; multiple eligibility routes available |
| Official Syllabus URL: | https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT |
ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Electrolytes, acid-base balance, and blood gases - Carbohydrates, lipids, proteins, and enzymes |
| Topic 2: Blood Banking (Immunohematology) | 15-20% | - Blood group systems and antigens - Compatibility testing - Antibody detection and identification - Blood components and storage - Transfusion reactions and adverse events |
| Topic 3: Urinalysis and Other Body Fluids | 5-10% | - Physical, chemical, and microscopic analysis - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing - Urine formation and composition |
| Topic 4: Microbiology | 15-20% | - Mycology, parasitology, and virology - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques - Identification of pathogenic microorganisms |
| Topic 5: Laboratory Operations | 5-10% | - Laboratory safety and regulatory compliance - Quality control and quality assurance - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes |
| Topic 6: Immunology and Serology | 5-10% | - Infectious disease serology - Immunologic testing methodologies - Immune system principles and components - Autoimmune and immunodeficiency disorders |
| Topic 7: Hematology | 20-25% | - Routine and automated hematology procedures - White blood cell morphology and diseases - Red blood cell physiology and disorders - Hemostasis and coagulation testing |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Which of the following disorders is characterized by increased production of chloride in sweat?
Multiple myeloma
A) Hypoparathyoidism
B) Down Syndrome
C) Cystic fibrosis
D) Wilson's disease
2. Francisella is slow growing on primary isolation culture media and produces poorly-staining coccobacilli on Gram stain. Because Francisella tularensis is included on the list of bioterrorism agents, suspicious isolates should be referred immediately to a public health laboratory in lieu of attempting an in-laboratory identification.
One of the BIGGEST problems with isolating Francisella is that these organisms:
A) produce wide zones of hemolysis
B) grow so slowly
C) produce greenish colonies like E. coli
D) overgrow other organisms
3. One method of calculating a glomerular filtration rate is using creatinine and urine volume to determine creatinine clearance. The equation is as follows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine; where urine flow rate = volume in mL /24 hours x h/60 min) In this case = creatinine clearance = 124 X (2200/24 x hour/60) / 2 = 94.7 or 95 ml/min What is the glomerular filtration rate for a patient with a serum creatinine of 2 mg/dL, if the urine creatinine was 124 mg/dL and the urine volume was 2.2 L/24 hrs?
A) 136 mL/min
B) 9.5 mL/min
C) 1.36 mL/min
D) 13.6 mL/min
E) 95 mL/min
4. Which method remains the "gold standard" for ANA detection?
A) Slide-based immunofluorescent assay (IFA) or Colorzyme
B) Radioimmunoassay (RIA)
C) Enzyme Immunoasay (ELISA)
D) Lateral Flow Immunoassay
5. Alkaline phosphatase, or ALP, is present in kidneys, liver, intestines, bone, and the placenta. The liver makes the largest amount of ALP. Some of the conditions associated with increased levels of ALP include: damaged liver cells, rapid bone growth (during puberty), bone diseases, or a disease that affects how much calcium is in the blood (hyperparathyroidism), and vitamin D deficiency.
Chemistry
Isoenzymes of alkaline phosphatase occur in:
A) Kidney, bone, intestines, liver
B) Liver, brain, spleen, intestines
C) Bone, brain, liver, pancreas
D) Brain, kidney, intestines, bone
Solutions:
| Question # 1 Answer: C | Question # 2 Answer: B | Question # 3 Answer: E | Question # 4 Answer: A | Question # 5 Answer: A |
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